Caspase-6 Fluorogenic Substrate

N° du produit ABIN924942

Aperçu rapide pour Caspase-6 Fluorogenic Substrate (ABIN924942)

Application: In vitro Assay (in vitro)

Détail du produit

Fonction: Peptide substrate labeled at the carboxy end with AFC (7-amino-4-trifluoromethyl coumarin). Designed to measure Caspase-6 activity in vitro.

Séquence: Ac-Val-Glu-Ile-Asp-AFC, Ac-VEID-AFC

Specificité: Highly specific substrate for Caspase-6. May be weak substrate for Caspases-1 and -3.

Information d'application

Indications d'application: For in vitro assays of Caspase-6 activity. Can be used with purified or partially purified enzymes or possibly with crude cell lysates (if the Caspase-6 Inhibitor is included to determine background protease activity).

Commentaires:

Fluorometer calibration: The fluorometer is calibrated using known concentrations of free AFC (Excitation = 400 nm, Emission = 505 nm) to generate a standard curve of fluorescence versus µ-moles AFC. Samples: Can be either purified or partially purified enzyme preparations. Application to crude cell lysates has not been confirmed. If crude cell lysates are to be assayed, the non- specific protease background must be determined using our Caspase-6 Inhibitor.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Détail du antigène

Sujet: Peptide substrate labeled at the carboxy end with AFC (7-amino-4-trifluoromethyl coumarin). Designed to measure Caspase-6 activity in vitro. Caspase-6 (also known as Mch2) is a member of the caspase family of cysteine proteases involved in apoptosis. It is a member of the Group III caspases (6, 8, and 9) which prefer the (L/V)EXD sequence as a substrate. Caspase-6 prefers a hydrophobic amino acid at P4, along with caspases-1 and -4, as opposed to the preference for Asp seen with caspases-2, -3, and -7. This is at odds with the gene sequence alignment that predicts Caspase-6 is more closely related to caspases-3 and -7 than to caspase-1. The preference by Caspase-6 for beta-branched amino acids in P4 fits well with the one known natural substrate, lamin A, and distinguishes it from caspases-1 and -4. Reconstitution experiments indicate that Caspase-6 activates caspases-3 and -7 and is therefore part of the proteolytic cascade that initiates apoptosis.

Poids moléculaire: 727.69 Da